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Medical, haematological and genetic determinants had been examined utilizing haemoglobin electrophoresis and allele-specific primers through polymerase sequence response to determine alpha and beta thalassemia, and CgT substitution at position -158 (known as Xmn-I polymorphism) in gamma-globin gene. Information was analysed utilizing SPSS 20. Eight b-thalassemia mutations were identified that included IVS I-5(G C), codon 8/9 (+G), codon 30 (G C), -88 (C T), Cap+1(A G), codon 41/42

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