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An 85% positive percentage of agreement was achieved, with only specimens with low viral loads being missed following 101 pooling. The average impact on both reverse transcription polymerase chain reactions within this assay was about 3 cycles. An 85% positive percentage of agreement was achieved, with only specimens with low viral loads being missed following 101 pooling. The average impact on both reverse transcription polymerase chain reactions within this assay was about 3 cycles.Aphis gossypii Glover (Hemiptera Aphididae) is a pol