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In both methods, GNFC/H2SO4 had the highest value followed by GNFC/NaOH, GNFC/NaClO and real sample nano fiber cellulose (RSNFC). The infrared spectral features showed no distinct changes of the four cellulose specimens at different conditions. The particle size distribution data proved that low acid concentration hydrolysis was not sufficient to obtain nano-sized cellulose particles. The Zeta potential was higher in accordance with (GNFC/H2SO4 GNFC/NaOH GNFC/NaClO), indicating the acid higher effect.Cardiovascular diseases (CVDs) are the leading