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The results demonstrated that the concentration of ammoniacal nitrogen in relation to AR treatments (69.; DF (44.; TF-AC (46.6) and TF-C (0.33) in mg/L can be a limiting factor for the development of embryos and larvae during the ecotoxicity tests. V.The increase of soil nitrogen (N) availability may alter soil microbial community composition and the natural N cycle in forest ecosystems. However, the responses of soil microbial nitrogen functional genes (NFGs) to N addition and their consequent effect on the N-cycle processes are poo