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Importance Many swimming bacteria generate a propulsion force by rotating helical filaments like propeller. However, the nonflagellated bacteria Spiroplasma swim without the use of the appendages. The tiny wall-less bacteria possess two chiral helices at a time, and the boundary called a kink travels down, possibly accompanying the dual rotations of the helices. To solve this enigma, we developed an assay to determine the handedness of the body helices at the single-wind level, and demonstrated that the co-existence of body helices trigg
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