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The strong impact of genotypes and their reaction on climatic elements in certain phenological stages were shown to be significant factors influencing the interactions among obligate pathogens and the predominance of one pathogen over another.Pseudomonas aeruginosa is known to play a role in many human diseases. Therefore, examining the negative control mechanisms of tight junction protein ZO-1 on the exotoxin LPS of P. aeruginosa-induced diseases could be critical in the development of novel therapeutics. We found that ZO-1 expression