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The determination of phosphoinositide molecular species in plant material is challenging because of their low abundance concurrent with a very high abundance of other membrane lipids, such as plastidial glycolipids. Phosphoinositides harbor an inositol headgroup which carries one or more phosphate groups at different positions of the inositol, linked to diacylglycerol via a phosphodiester. Thus, a further analytical challenge is to distinguish the different inositol-phosphate headgroups as well as the fatty acids of the diacylglycerol b
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