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During interactions with lynx1, an α4 C-loop tends become restricted in a choice of a closed or open condition, whereas the C-loop state transitions are more evident when lynx1 is unbound. Interestingly, the conformational change associated with the C-loop is stochastic, suggesting that lynx1 can influence nAChR (critical for its multimodal activity), by way of example, by moving its agonist sensitivity and recovery from desensitization.Atomic level deposition (ALD) on mechanically exfoliated 2D layer