https://www.selleckchem.com/pr....oducts/Rapamycin.htm
We have previously reported that heterozygous variation presented in platinum-resistant patients. This study aimed to further investigate the mechanism of mutation in the development of platinum resistance in ovarian cancer. The was synthesized and used to exchange 1 wildtype allele followed by sequencing to confirm the mutant allele sequence. Plasmids were constructed and transfected into the OVCAR-3 cells after lentiviral packaging. mRNA was detected by qPCR. BRCA2 protein was assessed by immunoblotting. Binding of the BRCA2 to Rad5
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