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Receiver operating characteristic curve analysis for VAS changes 15 minutes after 100 AU/mL challenge revealed that all nasal symptoms had area under the curve (AUC) values of ≥0.84 ( 0.001). TNSS change had an AUC value of 0.929 ( 0.001), while PNIF% change had an AUC value of 0.834. We could determine the optimal concentration (100 AU/mL), timing (15 minutes after challenge), and parameters (changes in TNSS and PNIF%) when performing NPT. We could determine the optimal concentration (100 AU/mL), timing (15 minutes after challe

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