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Nonetheless, low-temperature damage limits crop manufacturing. Genome of pitaya is not sequenced however. In this research, we sequenced the transcriptome of pitaya because the reference and further investigated the proteome under low-temperature. By RNAseq technique, about 25.3 million reads were acquired, and additional trimmed and assembled into 81,252 unigene sequences. The unigenes were looked against UniProt, NR and COGs at NCBI, Pfam, InterPro and Kyoto Encyclopedia of Genes and
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