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To raised control the use of fentanyl, it is crucial to build up fast and painful and sensitive detection techniques. In this study, an ultrasensitive monoclonal antibody (mAb) had been prepared and accustomed develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum. Under maximum conditions, the anti-fentanyl mAb from the subtype of Ig

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