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We therefore established the phylogenetic construction of the subglacial microbial communities making use of next generation sequencing (NGS) for the 16S rRNA gene and done bioinformatics analyses. An overall total of 1409 OTUs (working taxonomic units) belonged to 40 phyla; mainly Proteobacteria, Gracilibacteria, Bacteroidetes, Actinobacteria and Parcubacteria had been identified. Sites situated on the edge of Werenskioldbreen forefield (Angell, Kvisla) were mainly dominated by Bet