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First, we utilized volume-controlled (40 μL) paired DBS-whole blood examples to produce an analytical technique that involved the removal and measurement of MeHg and InHg with gasoline chromatography-cold vapor atomic fluorescence spectrometry (GC-CVAFS). Next, we completed a validation study using paired DBS-whole bloodstream samples from venous and capillary sources from 49 volunteers with Hg exposures much like background populations (i.e., MeHg in venous entire blood ranged from 0.