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Current RNA sequencing techniques are susceptible to arbitrary sampling restrictions as a result of complexity of this transcriptome and require considerable amounts of RNA material, specialized instrumentation, and high read counts to accurately interrogate A-to-I editing web sites. To address these challenges, we show that Escherichia coli Endonuclease V (eEndoV), an inosine-cleaving chemical, is repurposed to bind and isolate A-to-I edited transcripts from cellular RNA. While Mg2+ enables eEndoV

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