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05 for both). Testicular steroid signatures showed that both steroidogenic Δ and Δ pathways in the production of testosterone were activated more during prenatal development. Both 7α- and 11β-hydroxylations were predominant, while hydroxylations at C-6, C-15, and C-16 of testosterone and androstenedione were decreased in the fetus. The present GC-MS-based steroid profiling may facilitate understanding of the development of testicular steroidogenesis. Testicular steroid signatures showed that both steroidogenic Δ4 and Δ5 pathways in the

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