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In this respect, DNA polymerase θ differs off their Pols in that whereas purified Polθ misincorporates an A opposite 1,N6 -ethenodeoxyadenosine (εdA) making use of an abasic-like mode, Polθ executes predominantly error-free TLS in man cells. To try the hypothesis that Polθ adopts an alternate system for replicating through εdA in human cells than in the purified Pol, here we assess the effects of mutations in the two highly conserved tyrosine deposits, Y2387 and Y2391, when you look at the Polθ active
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