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Multiplex immunofluorescence analysis revealed that VISTA level was positively correlated with CD68 macrophages, CD3 T cells, and CD19 B cells in PDAC. However, a higher expression level of VISTA was detected in tumor-infiltrating CD68 macrophages than in CD3 T and CD19 B cells. Furthermore, anti-VISTA antibody treatment significantly reduced the number of metastatic nodules in livers of mouse models of PDAC with liver metastases. VISTA expressed in TCs is associated with a favorable prognosis in PDAC. Moreover, immunotherapy with anti-VIS
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