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Cancer treatment still faces big challenges in the clinic, which is raising concerns over the world. In this study, we report the novel strategy of combing bacteriotherapy with high-intensity focused ultrasound (HIFU) therapy for more efficient breast cancer treatment. The acoustic reporter gene (ARG) was genetically engineered to be expressed successfully in ( ) to produce the protein nanoparticles-gas vesicles (GVs). Ultrasound was utilized to visualize the GVs in . In addition, it was injected intravenously for targeted breast cancer