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It could then bring problems to anaerobic digestion as microplastics are great vector for toxic substances such as antibiotics and persistence organic pollutants. The key to determine the microplastics effect on anaerobic digestion is the desorption behavior of the toxic substances such as antibiotics, persistent organic pollutants and heavy metals from microplastics in digestion condition. Toxic compounds which are commonly presenting in sludge have shown the tendency to release from microplastics. It indicates that microplastics in sl