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is considered a dangerous pathogen, as it causes many human diseases, besides that it is resistant to almost all types of antibacterial agents. So, new strategies to overcome infection have evolved to attenuate its virulence factors and inhibit its quorum-sensing (QS) activity. This study investigated the effect of tyrosol and EDTA as anti-quorum-sensing and antivirulence agents against PAO1. Anti-quorum activity of sub-minimum inhibitory concentrations (sub-MICs) of tyrosol and EDTA was tested using (CV 12,472) biosensor bioassay. Mill
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