https://www.selleckchem.com/pr....oducts/fingolimod.ht
" We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9/gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency. Published under license by The American Society for Biochemistry and Molecular Biology, Inc.The ATPase Sec
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