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Radiographic determination of skeletal maturity may be used as a guide to the timing of bar removal following the correction of pectus excavatum. Radiographic determination of skeletal maturity may be used as a guide to the timing of bar removal following the correction of pectus excavatum.Single-cell RNA sequencing (scRNASeq) has advanced our understanding of lung biology, but its utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during

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