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The unusual involuntary motions scale assessed L-DOPA and dopamine D1- and D2-receptor agonist-induced dyskinesia. Lesions, verified via tyrosine hydroxylase and choline acetyltransferase immunohistochemistry reduced an average of 95% of nigral dopamine neurons and 80% of PPN cholinergic neurons, correspondingly. Rats receiving acetylcholine and double lesion demonstrated enhanced freezing, and acetylcholine lesioned rats exhibited increased printing location and stand index. Dopamin