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The stover composition from bmr12 35SSbF5H plants more closely resembled bmr12 stover than 35SSbF5H or wild-type (WT) stover; S-lignin and total lignin concentrations were decreased relative to WT or 35SSbF5H. Likewise, expression of upstream monolignol biosynthetic genes was increased in both bmr12 and bmr12 35SSbF5H relative to WT or 35SSbF5H. Overall, these results indicated that overexpression of SbF5H did not compensate for the loss of COMT activity. KEY MESSAGE Overexpression of F5H in sorghum increases S-lignin without increasing to
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