https://bms-582664inhibitor.co....m/aftereffect-of-gra
We produced in Escherichia coli Cdc8-tropomyosin bearing a phosphate group on Serine-125 (Cdc8 PS125), making use of an orthogonal tRNA-tRNA synthetase pair that right includes phosphoserine into proteins in reaction to a UAG codon when you look at the corresponding mRNA. We show utilizing complete internal expression (TIRF) microscopy that, whereas E.coli produced Cdc8 PS125 does not bind actin filaments, Cdc8 PS125 incubated with lambda phosphatase binds actin filaments. This work strai
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