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© 2021 Wiley Periodicals LLC. Basic Protocol 1 IVT with CleanCap Basic Protocol 2 mRNA purification and analysis.Mesenchymal stem cells (MSCs) are promising source of cell-based regenerative therapy. In consideration of the risk of allosensitization, autologous MSC-based therapy is preferred over allogenic transplantation in patients with chronic kidney disease (CKD). However, it remains uncertain whether adequate cell functionality is maintained under uremic conditions. As chronic inflammation and oxidative stress in CKD may lead to th
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