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Liver decellularisation ended up being done by portal vein perfusion using a detergent-based answer. Decellularised scaffolds were placed in a sterile perfusion device composed of a sealed organ chamber, operating at 37°C in normal atmospheric circumstances. The scaffold was perfused via portal vein with culture method. A total of 107 major cultured bone marrow stem cells, chosen by plastic adherence, were infused into the scaffold, after which it repopulated scaffolds had been perfused fo
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