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Stability and reusability for the immobilized preparations had been also evaluated. A noteworthy decolorization level (~90%) with immobilized HRP was accomplished. Phytotoxicity screening using Mung bean seeds and severe toxicity assay with Artemia salina has verified the applicability for the obtained immobilized preparation in commercial wastewater flowers for the treatment of colored wastewater. © 2020 American Institute of Chemical Engineers.BACKGROUND Bile acids tend to be multifaceted metabolic compounds tha