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We investigated the formation behavior of supported lipid bilayers (SLBs) on polyelectrolyte multilayers (PEMs) prepared using the layer-by-layer method. The SLBs were formed using the liposome fusion method, which was driven by electrostatic interactions. We used three types of cationic polyelectrolytes to prepare PEMs poly(diallyldimethylammonium chloride), which consists of a linear short polymer chain with quaternary ammonium cations, polyvinylamidin, which presents a linear, long polymer chain and polyethyleneimine which features a branched polymer cha