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Herein, label-free PGC-LC-MS/MS had been used to account with high quality the N- and O-glycome associated with real human monocyte-to-macrophage change. Main blood-derived CD14+-monocytes were classified ex vivo in the absence of powerful anti- and pro-inflammatory stimuli making use of the standard seven-day granulocyte-macrophage colony-stimulating element differentiation protocol with longitudinal sampling. Morphology and protein expression monitored by light microscopy and pr
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