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When salinity, temperature and pressure were set to seawater conditions, the values for interfacial tension between crude oil and water were below 1.0 mN m-1. Taken together, these results demonstrate that it is possible to obtain a nontoxic crude rhamnolipid product, with high productivity, to replace petroleum-based surfactants in oil spill cleanups and other environmental applications.In this research, the toxicological effect of untreated wastewater from of ethyl alcohol industry was evaluated on the zebrafish (Danio rerio) under experi
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