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During voyages, seafarers experience psychological problems that act to decrease operational safety. Psychological problems in seafarers can lead to changes in functional brain networks. This study investigated the low-frequency brain effective connectivity (EC) in seafarers during voyages by using the coupling strength (CS) of functional near-infrared spectroscopy (fNIRS) imaging. This study recruited 15 seafarers (seafarer group) working on a container ship and 15 healthy age-matched controls (control group). The EC was assessed using