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Species radiations have long served as model systems in evolutionary biology.1,2 However, it has only recently become possible to study the genetic bases of the traits responsible for diversification and only in a small number of model systems.3 Here, we use genomes of 36 species of North, Central, and South American warblers to highlight the role of pigmentation genes-involved in melanin and carotenoid processing-in the diversification of this group. We show that agouti signaling protein (ASIP) and beta-carotene oxygenase 2 (BCO2) are p