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In this work, we present an improved form of the plan that is on the basis of the same genetics and MLST database as Bom's MLST system, however with newly created primers for nested-1 and nested-2 tips under stringent circumstances. Additionally, we introduce a 3rd primer set for the sequencing step, which significantly gets better the overall performance for the assay. The enhanced primers had been tested in-silico using a dataset of 141 entire Genome Sequences (WGS) and in a comparative evaluat
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