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The optimum growth (μ), CO2 bio-fixation (RCO2) rates and the energy ratio (ER) of microalgae Chlorella vulgaris (C.v) were identified using central composite design statistical approach (CCD-SA). μ and RCO2 parameters including temperature of photobioreactor (TPBR), concentration of CO2 (CCO2 ), nutrients (carbon, nitrogen and phosphorus), gas flow rate (Qgas), initial inoculum concentration (INden) and the solar light intensity (Itot) were considered. Results revealed mild operational conditions in the range 20-25 °C, CCO2 of 2.5-20% (v
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