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Among the list of sleep, functional magnetic iron-oxide nanoparticles (Fe3O4 NPs) have shown great potential as an advanced system due to their built-in magnetic resonance imaging (MRI), biocatalytic task (nanozyme), magnetized hyperthermia therapy (MHT), photo-responsive therapy and drug distribution for chemotherapy and gene therapy. Magnetic Fe3O4 NPs can be synthesized through several practices and easily surface customized with biocompatible materials or energetic targeting moieties.