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T cells tend to be first isolated from peripheral bloodstream (right here leukoreduction system chambers) and a short while later activated for one day with anti-CD3/CD28 Dynabeads. The gene transfer is performed by lentiviral transduction and gene transfer price are validated by flowcytometric analysis. Six days after transduction, the stimulatory Dynabeads are eliminated. T cells are cultured in interleukin-2 conditioned medium for all days for development. There cl
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