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Since the use of amastigote forms within the indirect immunofluorescence assay has revealed an improvement into the specificity of this test when it comes to analysis of CVL, the performance of amastigotes forms of L. (L.) infantum chagasi as antigen resource were evaluated in automatized ELISA test using crude antigen of axenic amastigote and purified amastigote from spleen of hamster chronically infected researching with ELISA making use of total antigen created with promastigote forms of L. (L.) i
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