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Cultured C2C12 cells were incubated with a combination of indoxyl sulphate and p-cresol at high amounts (100 µg/mL) or low amounts (25 µg/mL and 10 µg/mL) resembling later or early CKD stages, respectively. Cell proliferation (analysed by scrape assays and flow cytometry) ended up being inhibited only by large doses of uraemic toxins, which inactivated the cdc2-cyclin B complex, suppressing mitosis and inducing apoptosis (analysed by annexin V staining). By contrast, low doses of uraemic toxins fail
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