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Saponins constitute an important class of secondary metabolites of the plant kingdom. Here, we present a mass spectrometry-based database for rapid and easy identification of saponins henceforth referred to as saponin mass spectrometry database (SMSD). With a total of 4196 saponins, 214 of which were obtained from commercial sources. Through liquid chromatography-tandem high-resolution/mass spectrometry (HR/MS) analysis under negative ion mode, the fragmentation behavior for all parent fragment ions almost conformed to successive losses of
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