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Thus, the targeting of OPN pathways seems to be a promising approach in the treatment of chronic liver diseases. INTRODUCTION AND OBJECTIVES Hepatocellular carcinoma (HCC) can recur following radiofrequency ablation and other hyperthermic treatment modalities. Cancer stem cells (CSCs) are a subpopulation of HCC cells that are difficult to eradicate and largely responsible for tumor recurrences. Thus, the principal objective of this study was to determine whether human HCC CSCs are relatively thermal-resistant compared to non-stem or matu