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Not surprisingly, compared to the Sil-PQn column only with quinine as practical team, the Sil-PQn-MIm column further modified by imidazolium ionic liquid possessed greater split performance, especially for the split of nucleosides/nucleobases. The consequences including buffer concentration, pH, organic solvent content and column temperature on chromatographic performance had been studied, which proved that multiple communications including electrostatic, hydrophobic and hydrophilic communications is simultaneously existed between y
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