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Here, we address the influence of rigidity on the lasting upkeep of pluripotency in real human induced pluripotent stem cells (hiPSCs) to determine whether soft substrates could provide an innovative new standard for hiPSC development and maintenance. To get this done, we set up a fabrication procedure of polyacrylamide-based culture supports with a rigidity-decoupled surface biochemistry. Soft elastic substrates with consistent and reproducible physicochemical properties had been design
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