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It is essential to control concentration gradients at specific locations for many biochemical experiments. This paper proposes a tunable concentration gradient generator actuated by acoustically oscillating bubbles trapped in the bubble channels using a controllable position based on the gas permeability of polydimethylsiloxane (PDMS). The gradient generator consists of a glass substrate, a PDMS chip, and a piezoelectric transducer. When the trapped bubbles are activated by acoustic waves, the solution near the gas-liquid interface is mixed. The vo

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