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The fungal secondary metabolite crude had been obtained from the mono and co-culture of Trichoderma spp. And had been examined by GC-MS, which was further subjected for antibacterial task against Xanthomonas oryzae pv. Oryzae, the causative organism for Bacterial Leaf Blight (BL in rice. Our results revealed that the most zone of inhibition was recorded through the co-culture of Trichoderma spp. rather than mono cultures, which shows that co-cultivatio