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42-123 h-1) to be used on the production of ASNase by this yeast. Results revealed that the enzyme production increased when using an initial cell concentration of 5.6 g L-1, mixture of sucrose and glycerol as carbon source, and k L a of 91.72 h-1. Under these conditions, the enzyme productivity was maximized, reaching 35.11 U L-1 h-1, which is already suitable for the development of scale-up studies. Additionally, accumulation of lipids was observed in all the cultivations, corresponding to 2-7 g L-1 (32-40% of the cell dry mass), with