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Organic carbon sources apportionment in river sediments is crucial to the output management of organic carbon. We conducted a source apportionment of sediment organic carbon in four rivers in Shaanxi Province, China, with a novel method that combined environmental scanning electron microscopy and energy dispersive X-ray spectrometry (ESEM-EDAX), principal component analysis (PCA), 16S rRNA sequencing, microbial community metabolic prediction, and positive matrix factorization (PMF). According to the ESEM-EDAX results, the sources of ligh