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Cell viability, apoptosis, invasion, and migration were dependant on MTT assay, circulation cytometry, transwell intrusion assay, and migration assay, correspondingly. Singlet oxygen generation ended up being recognized because of the singlet oxygen sensor green reagent assay. Our outcomes revealed that PDT with 5-ALA and GNPs-conjugated 5-ALA (5-ALA-GNPs) dramatically suppressed cell viability, increased cell apoptosis and singlet air generation in both HaCat and A431 cells, and PDT with 5-ALA and 5-AL