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This nonmedial slow division was influenced by an operating actomyosin band and vesicular trafficking, but independent of normal septum synthesis. Interestingly, the high intracellular turgor pressure appears to play a minor part in inhibiting band contraction within the lack of mobile wall surface renovating in cps1-191 mutants, as decreasing the turgor stress alone didn't enable spheroplast division. We suggest that during cytokinesis, the extracellular glycan matrix restricts actomyosin